Association Analysis of IL-1β, IL-6, and HMGB1 Variants with Postictal Serum Cytokine Levels in Children with Febrile Seizures and Genetic Epilepsy with Febrile Seizure Plus
Abstract number :
3.08
Submission category :
2. Translational Research / 2A. Human Studies
Year :
2019
Submission ID :
2421979
Source :
www.aesnet.org
Presentation date :
12/9/2019 1:55:12 PM
Published date :
Nov 25, 2019, 12:14 PM
Authors :
Ji Eun Choi, Seoul National University Boramae Medical; Soo Yeon Kim, Seoul National University Children's Hospital; Hunmin Kim, Seoul National University - Bundang Hospital; Byung Chan Lim, Seoul National University; Hee Hwang, Seoul National University
Rationale: Febrile seizures (FS) is the unique type of seizures only occurred during early childhood. Genetic epilepsy with febrile seizure plus (GEFS+) is familial epilepsy syndrome associated with FS and afebrile seizures and show diverse genetic heterogeneity. Both seizure types are related with fever provocation, however whether genetic susceptibility to inflammation is implicated in FS and GEFS+ is still unclear. The aim of the study was to analyze an association between postictal serum cytokine levels and genetic polymorphisms in cytokines genes of IL-1β, IL-6 and HMGB1 in FS and GEFS+. Methods: Fifty-seven FS and 43 GEFS+ were included. Blood was obtained within 2 hours after febrile seizures in FS and afebrile seizures in GEFS+. Controls were 108 febrile children without FS. Serum IL-1β, IL-6 and HMGB1 were analyzed with ELISA. Genotyping was performed with SNaPshot for IL-1β-31 (rs1143627), -511 (rs16944), IL-6-572 (rs1800796) and HMGB1 3814 (rs2249825). The allelic, genotypic distribution and correlations with serum cytokine levels were analyzed. Results: For IL-1β-31 and -511, CT genotypes showed significantly higher postictal serum IL-1β than CC+TT among FS (17.2 vs 7.4 pg/ml, both p <
Translational Research