Abstracts

Rationale: Most of current treatments of epilepsy aimed to remove epileptogenic zone, inhibit the propagation of epileptiform discharge, and/or improve the kindling threshold value, and the therapeutic theory for epilepsy is to inhibition seizure. We wanted to confirm that conduction of the epileptiform discharge activity out of the cranium can by reduce the seizure and consequence neuron injury, and introduce the novel micro invasive neurosurgical approach to epileptic therapy.Methods: To build penicillin-induced neocortical epilepsy rat. The self-made conducting electrode consisted of a head platinum patch with 5mm in diameter with 1 insulated side and 1 naked side, and an end platinum patch with 5mm in diameter with both naked sides. There is an insulation lagging covered connecting platinum silk 15mm in length between two patches. However, the end patch of pseudo-leading electrode is with both insulated sides. The specialized self-made conducting electrode was used to building conducting epileptic rat model. The naked side of head patch was planted on the surface of neocortex, and the end patch was planted under skin of neck. The epileptiform discharges were recorded by EEG with deep needle electrode for 2 hours under anesthesia, and seizures were monitored by video for 48 hours in waking. At last, the apoptosis ratio of neocortex was tested with flow cytometer. Results: The mean frequency of epileptiform discharges and frequency of diffused epileptiform discharges in EEG in conducting group, which were 4.21 3.76 and 1.86 1.88, were significantly less than the numbers in model control group and pseudo-conduction group(p<0.01). However, significant difference was not found in times of focal epileptiform discharges among 3 test groups. During video monitoring, significant difference presented in the frequency of clinical seizure between conducting group and model control group or pseudo-conducting group (p<0.01). .Furthermore, apoptosis ratios of neuron in neocortex were 4.01 1.43 in conducting group, 10.12 2.62 in model control group, and 10.56 3.19 in pseudo-conducting group, and the apoptosis ratio of neuron in conducting group was significantly less than the other two groups (p<0.01). Conclusions: Conducting the epileptiform discharge activity out of the cranium can prevent the seizure and reduce epileptiform discharge and apoptosis ratio of neocortex in neocortical epilepsy rats.