Genome-wide long non-coding RNA analysis in mouse models of temporal lobe epilepsy
Abstract number :
1.018
Submission category :
1. Translational Research: 1A. Mechanisms / 1A1. Epileptogenesis of acquired epilepsies
Year :
2016
Submission ID :
194992
Source :
www.aesnet.org
Presentation date :
12/3/2016 12:00:00 AM
Published date :
Nov 21, 2016, 18:00 PM
Authors :
Byoung-su Park, Seoul National University Hospital; Jangsup Moon, Seoul National University Hospital; Jung-Ah Lim, Seoul National University Hospital; Jin Sun Jun, Seoul National University Hospital; Tae-Won Yang, Seoul National University Hospital; Keun
Rationale: Long non-coding RNAs (LncRNAs) have received increasing attention for their capacity to modulate epigenetic regulation and their suggested role in various human diseases. Temporal lobe epilepsy is the most common form of partial epilepsy, and epigenetic regulations may take part in the process of epileptogenesis. However, comprehensive profiling of lncRNAs in temporal lobe epilepsy has been limited. Methods: We used pilocarpine and kainite models. lncRNAs were analyzed by microarray (Agilent SurePrint G3 Mouse GE 8x60K Microarray) using entire brains. Subssequently, we performed more extensive profiling of lncRNAs using Arraystar Mouse LncRNA Expression Microarray V3.0 in the mouse pilocarpine models according to the brain regions and compared them to the control mouse. Results: A total of 4,622 lncRNAs were analyzed by microarray using entire brains: 384 lncRNAs were significantly dysregulated in pilocarpine model, and 279 lncRNAs were significantly dysregulated in kainate model compared with control mice (?-3.0-fold, P< 0.05). Among these, 54 and 14 lncRNAs, respectively, had adjacent protein-coding genes whose expressions were also significantly dysregulated (?-2.0-fold, P< 0.05). Hippocampus and cortex, the two main structures of the brain involved in the process of epileptogenesis, were used for more extensive profiling of lncRNA. A total of 35,923 LncRNAs were analyzed. As a result, 22 and 83 LncRNAs were up- and down-regulated (?-2.0-fold, P< 0.05) in the hippocampus of the epilepsy models, while 46 and 659 LncRNAs were up- and down-regulated in the cortex of the epilepsy models. Among these, 5 IncRNAs were up-regulated and 14 were down-regulated in both hippocampus and cortex of the epilepsy models Conclusions: This is the first study to demonstrate the profiles of dysregulated LncRNAs in chronic epilepsy model. We believe that this finding will serve as a stepping stone in the emerging LncRNA research field. Funding: None
Translational Research