Abstracts

Rationale: Reactive oxygen intermediates can damage various forms of biomolecules, such as DNA, lipids, and proteins in human. Urinary and cerebrospinal fluid (CSF) levels of 8-hydroxydeoxyguanosine (8-OHdG) were examined the relevancy of oxidative stress to the disease in children with prolonged febrile seizures (PFS). Methods: Urinary (U) and CSF 8-OHdG levels were measured in 15 children with PFS and in healthy children. The relationship between U and CSF levels of 8-OHdG was determined in 5 children where both U and CSF samples were available. The measurement of U 8-OHdG was conducted with an ELISA Kit and CSF 8-OHdG was analyzed by a computer-controlled HPLC analyzer. Results: The levels of U and CSF 8-OHdG in children without brain damage associated with PFS showed no significant deference compared to healthy children. In contrast, the levels of U and CSF 8-OHdG in children with brain damage associated with PFS were significantly higher than that of healthy children. A positive correlation between the levels of U and CSF 8-OHdG was noted in 5 children with both U and CSF samples were available. Conclusions: These results suggest that oxidative stress was strongly related to the acute brain damage in children following PFS. We disclose that U and CSF 8-OHdG was a useful marker of oxidative stress in children with brain damage associated with PFS.